| Specification
sheet |
Tumor
Necrosis Factor alpha,
Recombinant
Human (TNFa)
|
| Catalog
No: PC110-1 |
(10 micrograms) |
$115.00 |
| Catalog
No: PC110-2 |
(50 micrograms) |
$211.00 |
| Catalog
No: PC110-3 |
(1 mg) |
CALL/EMAIL |
| Source |
E.Coli |
| Formulation |
The protein was lyophilized after extensive
dialysis against 5 mM Tris buffer pH 8
|
| Storage |
Lyophilized
Recombinant Human TNF-alpha, although stable at room temperature,
should be stored desiccated below 0 degree Celsius. Reconstituted
Recombinant Human TNF-alpha is best stored refrigerated at 4
degree Celsius. |
| Purity |
Greater
that 99.0% determined by
a) Analysis by RP-HPLC
b) anion-exchange FPLC
c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained. |
| Biological
Activity |
Recombinant
Human TNF-alpha is fully biologically active when compared to
standard. The ED50 as determined by the
cytolysis of murine L929 cells in the presence of Actinomycin
D is <= 0.05ng/mL, corresponding to a Specific Activity of
2.2x10^7 IU/mg. |
| Description |
Recombinant
Human TNF-alpha produced in E.Coli is a single, non-glycosylated,
polypeptide chain containing 158 amino acids and having a molecular
mass of 17483.77 Dalton. The Recombinant Human TNF-alpha is
purified b y standard chromatographic techniques. |
| Endotoxcin |
Less than
or equal to 0.1ng/ug (IEU/ug) |
| Physical
Appearance |
Sterile
filtered white lyophilized (freeze-dried) powder. |
| Solubility |
The lyophilized
TNF-alpha is very soluble in water and most aqueous buffers
below and above isoelectric point (pI-7.44). |
| Amino
Acid Composition |
In total
agreement with the expected amino acid composition of native
human TNF-alpha. |
| Amino
Acid Sequence |
The sequence
of the first five N-terminal amino acids was determined and
was found to be Met-Val-Arg-Ser-Ser, confirming the sequence
of native human TNF-alpha. |
| OCT
USA Tel:
1-800-771-7001 / 1-310-533-8704
Fax: 1-310-533-8720 E-mail:
info@octusa.com
|